The experiment studied the change of non-specific immune in rising temperature (16℃, 20℃, 24℃,28℃). The activities of CAT, SOD, POD, LSZ and the content of MDA in Shuiyuan NO.1 sea cucumber and Dalian cultured sea were analyzed. The result show that: (1) in rising temperature,the change of CAT, SOD, POD were trend to increased and to decreased in Shuiyuan NO.1 sea cucumber and Dalian cultured sea cucumber, the activities of CAT were 77.160-151.76 U/mL and 24.617-51.740 U/mL, the activities of SOD were 0.070-0.154 U/mL and 0.056~0.100 U/mL, the activities of POD were 2.360-3.470 U/mL and 1.407-2.407 U/mL. The change of LSZ was first increased and then decreased and increased, the activities of LSZ were: 9.741-30.898 U/mL and 8.554-28.006 U/mL. The content of MDA in Shuiyuan NO.1 sea cucumber was trend to increased, the change of MDA was 42.331-46.563 nmol/mL.The content of MDA in Dalian cultured sea cucumber was trend to decreased and to increased, the change was 46.079-47.329 nmol/mL.(2) In rising temperature, the change of CAT, SOD, POD, LSZ in Shuiyuan NO.1 sea cucumber were more sensitive than in Dalian cultured sea cucumber, and the change of CAT and POD had significant difference (P <0.05). In 28℃, the content of MDA in Shuiyuan NO.1 sea cucumber was significantly less than in Dalian cultured sea cucumber (P <0.05). The study indicated that Apostichopus japonicus (Selenka) could make certain physiological immune response to elevated temperatures, to rid the body of excess free radicals by regulating the immune defense enzyme activity, to protect body from damage caused by environmental change. Shuiyuan NO.1 sea cucumber was more sensitive and had hight adaptation in high temperature than Dalian cultured sea cucumber.
The experiment was important to study the high temperature adaptation ofApostichopus japonicus (Selenka) and to promote Shuiyuan NO.1 sea cucumber.