To clarify the effect of compound fertilizer reduction with bio-organic fertilizer, taking a tomato cultivar ‘Tuteng’ as the study object, we studied the effects of applying bio-organic fertilizer combined with different compound fertilizer reduction (10%, 20% and 30%) on soil fertility and enzyme activity. The results showed that: compared with conventional fertilization (CK), chemical fertilizer reduction combined with bio-organic fertilizer could significantly increase the soil available nutrients, compared with compound fertilizer (TK) treatment, the contents of soil organic matter, balkali-nitrogen, available phosphorus and available potassium increased by 6.42%-26.11%, 14.75%-26.23%, 4.38%-10.74% and 26.63%-39.09%, respectively; compared with CK and TK, chemical fertilizer reduction combined with bio-organic fertilizer significantly decreased soil enzyme activity during seedling period; and increased the activities of urease and catalase at flowering stage by 73.05%-92.74% and 14.65%-32.23%, respectively; during maturity, the bio-organic fertilizer combined with chemical fertilizer reduction 20% (F2) increased the urease activity by 10.90%, and the bio-organic fertilizer combined with chemical fertilizer reduction 10%, 20%, 30% (F1, F2, F3) significantly increased the catalase activity by 55.56%, 82.22% and 76.67%, respectively; with the extension of the growth period of tomato, the catalase activity increased first and then decreased.