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农学学报 ›› 2022, Vol. 12 ›› Issue (12): 1-5.doi: 10.11923/j.issn.2095-4050.cjas2021-0143

• 农艺科学/生理生化 •    下一篇

F型与其他小麦雄性不育系细胞质类型的分子鉴定

任文斌(), 王倩, 吴翠翠, 谢三刚   

  1. 山西农业大学(山西省农业科学院)棉花研究所,山西运城 044000
  • 收稿日期:2021-08-25 修回日期:2021-11-05 出版日期:2022-12-20 发布日期:2023-01-05
  • 作者简介:任文斌,男,1980年出生,山西沁源人,副研究员,硕士,主要从事小麦遗传育种研究。通信地址:044000 山西省运城市黄河大道118号 山西省农科院棉花研究所,E-mail:renwenbin1980@163.com
  • 基金资助:
    山西省重点研发计划重点项目课题“F型或其它不育系繁制种技术研究”(201903D211004-1-2)

F Type and Other Male Sterile Lines of Wheat: Molecular Identification of Cytoplasmic Types

REN Wenbin(), WANG Qian, WU Cuicui, XIE Sangang   

  1. Institute of Cotton Research, Shanxi Agricultural University(Shanxi Academy of Agricultural Sciences), Yuncheng 044000, Shanxi, China
  • Received:2021-08-25 Revised:2021-11-05 Online:2022-12-20 Published:2023-01-05

摘要:

为了鉴定F型与其他小麦雄性不育系细胞质类型之间差异。以F型不育系(FA1-1,FA1-2,FA2-1)、F型保持系、T、K、V型同核异质不育系(A)及其保持系(B)‘冀5418’等为试验材料,用23对叶绿体和21对线粒体SSR分子标记进行聚类分析。结果表明:FA与T、K、V、B型不育系的细胞质分属不同类别。FA与中国春(CS)的细胞质遗传相似系数为1.00,FA与FB细胞质遗传相似系数为1.00,表明其可能为普通小麦细胞质雄性不育系。与经典质核互作雄性不育原理有差异,F型雄性不育系的育性可能由核基因控制。

关键词: 小麦, F型雄性不育系, 叶绿体, 线粒体, SSR

Abstract:

In order to identify the difference of cytoplasmic types between F type and other male sterile lines of wheat, the experiment was conducted with F type sterile line (FA1-1, FA1-2, FA2-1), F type maintainer line, the T, K and V type CMS lines (A) and the common maintainer line (B) ‘Ji5418’ as the materials, and 23 pairs of chloroplasts and 21 pairs of mitochondrial SSR markers were used for cluster analysis. The results showed that the cytoplasm of FA and T, K, V and B male sterile lines belonged to different categories. The cytoplasmic genetic similarity coefficient between FA and Chinese Spring (CS) was 1.00, and the cytoplasmic genetic similarity coefficient between FA and FB was also 1.00, indicating that FA might be a cytoplasmic male sterile line of common wheat. The fertility of F type male sterile line might be controlled by nuclear genes, which is different from the classical cytoplasmic-nuclear interaction male sterile principle.

Key words: wheat, F type male sterile line, chloroplast, mitochondria, SSR

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