亚硝酸钠-硝酸铝比色法测定干旱胁迫前后玉米幼苗的总黄酮含量

doi:10.11923/j.issn.2095-4050.cjas20190800161

摘要/Abstract

摘要：

为研究干旱胁迫对不同品种玉米幼苗总黄酮含量的影响,采用亚硝酸钠-硝酸铝比色法,对干旱胁迫前后'浚单20'、'郑单958'和'豫玉22'玉米幼苗的总黄酮含量进行测定,并对试验方法进行方法学考察。结果表明,3个品种玉米幼苗在干旱胁迫前总黄酮含量从高到低依次为'浚单20'、'郑单958'、'豫玉22',干旱胁迫后总黄酮含量从高到低依次为'郑单958'、'浚单20'、'豫玉22'。干旱胁迫后3个品种玉米幼苗总黄酮含量均有所提升,其中'郑单958'在干旱胁迫后总黄酮含量提升最多,为16.21%,'浚单20'其次,为7.61%,'豫玉22'提升较少,为2.67%。亚硝酸钠-硝酸铝比色法测定玉米幼苗总黄酮含量时,其精密度、稳定性和加标回收率均符合方法学要求,具有良好的可行性。干旱胁迫前后,不同品种玉米幼苗由于自身对干旱的敏感程度不同,其总黄酮含量有不同程度的响应。本试验可为研究干旱胁迫下不同品种玉米次生代谢产物总黄酮含量的变化提供一定的参考依据。

关键词: 玉米, 干旱胁迫, 亚硝酸钠-硝酸铝比色法, 总黄酮含量

Abstract:

To study the effects of drought stress on the total flavonoids content of different maize seedlings, sodium nitrite-aluminum nitrate colorimetric method was used to determine the total flavonoid of 'Xudan 20', 'Zhengdan 958' and 'Yuyu 22' maize seedlings before and after drought stress, and the method was verified with methodology. Results showed that the total flavonoids contents in maize seedlings of the three varieties from high to low was 'Xundan 20', 'Zhengdan 958', 'Yuyu 22' before drought stress, and it changed to be 'Zhengdan 958', 'Xundan 20', 'Yuyu 22' after drought stress. The total flavonoids contents in maize seedlings of the three varieties increased after drought stress, 'Zhengdan 958' increased the most (16.21%), followed by 'Xundan 20' (7.61%), and the lowest was in 'Yuyu 22' (2.67%). The precision, stability and recovery of the sodium nitrite-aluminum nitrate colorimetric method used in the study were conformed to the requirement of methodology, showed good feasibility. Before and after drought stress, the total flavonoids of different varieties had different response due to their distinct sensitivity. This experiment could provide certain references for studying the changes of total flavonoids in secondary metabolites of different maize varieties under drought stress.

Key words: Maize, Drought Stress, Sodium Nitrite-Aluminum Nitrate Colorimetric Method, Total Flavonoids Content

中图分类号:

S132

任园宇, 魏东伟, 王中伟, 周亚萍. 亚硝酸钠-硝酸铝比色法测定干旱胁迫前后玉米幼苗的总黄酮含量[J]. 农学学报, 2020, 10(5): 15-20.

Ren Yuanyu, Wei Dongwei, Wang Zhongwei, Zhou Yaping. Total Flavonoids in Maize Seedlings Before and After Drought Stress: Determination with Sodium Nitrite-Aluminum Nitrate Colorimetry[J]. Journal of Agriculture, 2020, 10(5): 15-20.

图/表 8

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项目	样品液1	样品液2	样品液3	样品液4	样品液5	样品液6
A₅₀₈/a.u.	0.686	0.712	0.695	0.686	0.645	0.682
总黄酮含量/(mg/g)	51.79	53.91	52.52	51.79	48.46	51.47

项目	样品液1	样品液2	样品液3	样品液4	样品液5	样品液6
A₅₀₈/a.u.	0.686	0.712	0.695	0.686	0.645	0.682
总黄酮含量/(mg/g)	51.79	53.91	52.52	51.79	48.46	51.47

项目	0 h	0.5 h	1 h	1.5 h	2 h	2.5 h	3 h	3.5 h
A₅₀₈/a.u.	0.673	0.671	0.655	0.640	0.640	0.659	0.669	0.707
总黄酮含量/(mg/g)	50.73	50.57	49.27	48.05	48.05	49.59	50.41	53.50

项目	0 h	0.5 h	1 h	1.5 h	2 h	2.5 h	3 h	3.5 h
A₅₀₈/a.u.	0.673	0.671	0.655	0.640	0.640	0.659	0.669	0.707
总黄酮含量/(mg/g)	50.73	50.57	49.27	48.05	48.05	49.59	50.41	53.50

样品		样品测定值/(mg/g)	加标量/(mg/g)	加标样品测定值/(mg/g)	回收率/%	平均回收率/%
干旱组	浚单20	53.50	10	64.63	101.80	102.29
	郑单958	46.67	10	57.72	100.86
	豫玉22	43.17	10	55.12	103.68
对照组	浚单20	48.86	10	60.00	101.93
	郑单958	42.52	10	53.65	102.35
	豫玉22	41.71	10	52.92	102.37