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农学学报

农学学报 ›› 2013, Vol. 3 ›› Issue (3): 44-46.

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

春兰‘金荷鼎’杂交后代离体快繁的研究

牛田 张林 李承秀 孙芳 于永畅 颜婷美 王长宪   

  • 收稿日期:2012-12-21 修回日期:2013-02-28 出版日期:2013-03-20 发布日期:2013-03-20
  • 基金资助:
    国家公益专项“国兰新品种培育与产业化生产关键技术研究”

Study on Filial Generation of Cymbidium goeringii ‘Jinheding’ in in vitro Propagation

  • Received:2012-12-21 Revised:2013-02-28 Online:2013-03-20 Published:2013-03-20

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摘要： 以‘金荷鼎’ב赤蕙’根状茎为材料，研究根状茎的增殖情况，包括基本培养基的筛选、6-BA与NAA的浓度组合对增殖的影响。不同培养基对根状茎生长情况有明显影响。1/2MS培养基为春兰根状茎的基本培养基，1/2MS+NAA 1 mg/L+6-BA 0.5 mg/L为春兰根状茎的最佳增殖培养基，增殖倍数达3.00。杂交育种技术结合组织培养是国兰新品种选育的有效途径。

关键词: 烤烟, 烤烟, 方差分析, AMMI模型, GGE双标图, 品种评价

Abstract: Taking ‘Jinheding’ and ‘Chihui’ as parents, rootstocks were used as materials for studying the multiplication of rootstocks. There were including choices of basic medium, the effects of 6-BA and NAA on the multiplication. The result showed that the growth state was affected evidently by medium. 1/2MS medium was the basic medium for rootstocks of Cymbidium goeringii. 1/2MS+ NAA 1 mg/L+6-BA 0.5 mg/L was the best medium for multiplication. proliferation rate was 3.00. Cross breeding technology combining with tissue culture was the effective way for the new species breeding of Cymbidium.