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农学学报 ›› 2019, Vol. 9 ›› Issue (1): 59-61.doi: 10.11923/j.issn.2095-4050.cjas18030005

所属专题: 园艺

• 农业工程 农业机械 生物技术 食品科学 • 上一篇    下一篇

金银花茎、叶和花中化学成分的比较研究

章艳玲   

  1. 新乡学院 化学化工学院
  • 收稿日期:2018-03-06 修回日期:2018-04-17 接受日期:2018-04-24 出版日期:2019-01-18 发布日期:2019-01-18
  • 通讯作者: 章艳玲 E-mail:zhang2002040
  • 基金资助:
    河南省教育厅科学技术研究项目“金银花茎、叶和花的有效成分比较研究”(2011C180003)。

A Comparison of Chemical Constituents in Different Parts of Honeysuckle

  • Received:2018-03-06 Revised:2018-04-17 Accepted:2018-04-24 Online:2019-01-18 Published:2019-01-18

摘要: 为了比较金银花茎、叶和花中的化学成分含量,采用了硫酸-蒽酮法、苯酚-硫酸法、2,6-二氯酚靛酚滴定法、考马斯亮蓝法、茚三酮呈色法和紫外分光光度法,分别测定了金银花茎、叶和花中的黄酮类物质、维生素C、绿原酸、可溶性蛋白质、游离氨基酸、可溶性糖和多糖的含量。结果表明:黄酮类化合物和维生素C含量均为叶>花>茎;而绿原酸、可溶性蛋白质和游离氨基酸含量均为:花>叶>茎,虽然花中含量最高,但叶和茎中也有一定的含量;可溶性糖:茎>花>叶;多糖含量:茎>叶>花。这为金银花的综合利用提供了一定的理论依据。

关键词: 油菜, 油菜, 花期, 预测模型, 高寒, 相关分析

Abstract: This study aims to compare the chemical constituents in stems, leaves and flowers of honeysuckle. The contents of flavonoids, vitamin C, chlorogenic acid, soluble protein, free amino acid, soluble sugar and polysaccharide are measured by sulfuric acid- anthrone method, phenol- sulfuric acid method, 2,6- di chlorphenol indophenol titration method, Coomassie brilliant blue method, ninhydrin coloration method, and ultraviolet spectrophotometry. Results showed that the content of flavonoids and vitamin C in honeysuckle organs were leaf > flower > stem, the content of chlorogenic acid, soluble protein and free amino acid in honeysuckle organs were flower > leaf > stem, although the content in flower was the highest, there were also certain contents in leaves and stems; the soluble sugar content in honeysuckle organs was stem > flower > leaf, and the polysaccharide content was stem > leaf > flower. This study provides a basis for comprehensive utilization of honeysuckle.