双氧水与PEG协同引发对小麦种子活力的影响

doi:10.11923/j.issn.2095-4050.cjas2021-0213

摘要/Abstract

摘要：

为了解双氧水与PEG协同引发对小麦种子活力的影响,测定了引发处理后冬小麦的苗长(SL)、根长(RL)、发芽势(GE)、发芽率(GP)等9个种子活力指标。结果表明,‘长7170’在0.25%H₂O₂浸泡6 h+10%PEG浸泡4 h（处理3）条件下比对照（处理1）的发芽势、发芽指数、苗长、活力指数显著升高了18.4%、30.2%、26.1%、48.4%,对比于0%H₂O₂浸泡12 h+0%PEG浸泡12 h（处理2）的发芽指数、活力指数、根长、根重分别升高了20.7%、16.2%、13.6%、22.8%。‘长6388’在0.25%H₂O₂浸泡6 h+10%PEG浸泡4 h（处理3）条件下的发芽率显著高于对照（处理1）及0.5%H₂O₂浸泡6 h+5%PEG浸泡4 h（处理9）,发芽指数与0.5%H₂O₂浸泡12 h+10%PEG浸泡2 h（处理8）相比升高了12%。种子活力的综合评价结果（D值）显示,2个品种均在处理3的协同引发下最大,分别为0.9482、0.8293;GGE双标图分析表明,0.25% H₂O₂浸泡6 h+10%PEG浸泡4 h（处理3）协同引发下,2个品种都在发芽势、发芽率、发芽指数、活力指数和根长上表现良好,这些指标可以作为小麦种子活力鉴定的主要指标。

关键词: 小麦, 双氧水, PEG, 协同引发, 种子活力

Abstract:

In order to understand the effect of synergistic initiation of hydrogen peroxide and PEG on seed vigor, nine seed vigor indexes including seedling length (SL), root length (RL), germination potential (GE) and germination percentage (GP) and etc. of winter wheat cultivars after initiation were measured. The results showed that under the condition of 0.25%H₂O₂ soaking for 6 h and 10%PEG soaking for 4 h (treatment 3), the germination potential, germination index, seedling length and vigor index of ‘Chang 7170’ significantly increased by 18.4%, 30.2%, 26.1% and 48.4%, respectively, compared with those of the control (treatment 1); and the germination index, vigor index, root length and root weight increased by 20.7%, 16.2%, 13.6% and 22.8%, respectively, compared with those under 0%H₂O₂ soaking for 12 h and 0%PEG soaking for 12 h (treatment 2). The germination rate of ‘Chang 6388’ under 0.25%H₂O₂ soaking for 6 h and 10%PEG soaking for 4 h (treatment 3) was significantly higher than that of the control (treatment 1) and 0.5%H₂O₂ soaking for 6 h and 5%PEG soaking for 4 h (treatment 9); and the germination index increased by 12% compared with that under 0.5%H₂O₂ soaking for 12 h and 10%PEG soaking for 2 h (treatment 8). The comprehensive evaluation results (D value) showed that the seed vigor of the two cultivars was the highest under the synergistic initiation of treatment 3, which was 0.9482 and 0.8293, respectively. GGE biplot analysis showed that the germination potential, germination rate, germination index, vigor index and root length of the two cultivars were all good under the synergistic initiation of 0.25% H₂O₂ soaking for 6 h and 10%PEG soaking for 4 h (treatment 3), which could be used as the main indexes for identifying the vigor of wheat seeds.

Key words: wheat, hydrogen peroxide, PEG, synergistic initiation, seed vigor

中图分类号:

S512.1

马晔, 杨进文, 李宁, 范李剑, 史雨刚. 双氧水与PEG协同引发对小麦种子活力的影响[J]. 农学学报, 2022, 12(4): 6-12.

MA Ye, YANG Jinwen, LI Ning, FAN Lijian, SHI Yugang. Synergistic Initiation Effect of Hydrogen Peroxide and PEG on Wheat Seed Vigor[J]. Journal of Agriculture, 2022, 12(4): 6-12.

图/表 9

参考文献 21

[1]	曹丹, 白耀博, 强承魁, 等. 不同品种小麦种子萌发对镉胁迫的耐性响应[J]. 黑龙江农业科学, 2017(7):8-11.
[2]	郭程, 曾占奎, 韩志鹏, 等. 小麦种子活力相关性状研究进展[J]. 中国种业, 2021, 317(8):30-33.
[3]	荆文旭, 魏磊, 万雪, 等. 引发对自然老化甜菜种子活力及幼苗生理特性的影响[J]. 种子, 2021, 40(8):116-121,126.
[4]	BRITS G J, BROWN N A C, CALITZ F J, et al. Effects of storage under low temperature, room temperature and in the soil on viability and vigour of Leucospermum cordifolium (Proteaceae) seeds[J]. South African journal of botany, 2015, 97:1-8. doi: 10.1016/j.sajb.2014.11.003 URL
[5]	VENTURA L, DONà MATTIA, MACOVEI A, et al. Understanding the molecular pathways associated with seed vigor[J]. Plant physiology and biochemistry, 2012, 60:196-206. doi: 10.1016/j.plaphy.2012.07.031 URL
[6]	李洁, 徐军桂, 林程, 等. 引发对低温胁迫下不同类型玉米种子萌发及幼苗生理特性的影响[J]. 植物生理学报, 2016, 52(2):157-166.
[7]	冯燕茹, 周琪, 李嵩, 等. 不同小麦品种(系)的存放种子活力比较研究[J]. 种子, 2019, 38(1):5-12.
[8]	夏方山, 董秋丽, 毛培胜, 等. PEG引发对老化燕麦种胚细胞与线粒体结构及抗氧化性能的影响[J]. 草业学报, 2018, 27(5):170-177.
[9]	姚东伟, 吴凌云, 沈海斌, 等. 种子引发技术研究与应用进展[J]. 上海农业学报, 2020(5):153-160.
[10]	FAROOQ S, HUSSAIN M, JABRAN K, et al. Osmopriming with CaCl2 improves wheat (Triticum aestivum L.) production under water-limited environments[J]. Environmental science & pollution research, 2017, 24(15):1-12.
[11]	KRóL P, IGIELSKI R, POLLMANN S, et al. Priming of seeds with methyl jasmonate induced resistance to hemi-biotroph Fusarium oxysporum f. sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation[J]. Journal of plant physiology, 2015, 179:122-132. doi: 10.1016/j.jplph.2015.01.018 URL
[12]	MAjDA C, D KHALID, AZIZ A, et al. Nutri-priming as an efficient means to improve the agronomic performance of molybdenum in common bean (Phaseolus vulgaris L.)[J]. Science of the total environment, 2019, 661(APR.15):654-663. doi: 10.1016/j.scitotenv.2019.01.188 URL
[13]	刘天丽, 付炳堃, 李晓, 等. 引发对干旱胁迫下菠菜种子萌发的影响[J]. 种子, 2019, 38(1):86-89.
[14]	LI Z, GAO Y, ZHANG Y C, et al. Reactive Oxygen Species and Gibberellin Acid Mutual Induction to Regulate Tobacco Seed Germination[J]. Frontiers in plant science, 2018,9.
[15]	张珂, 厉萌萌, 刘德权, 等. 镉胁迫对小麦、玉米种子萌发及幼苗生长的影响[J]. 种子, 2019, 38(5):90-94.
[16]	王瑛. 促进特殊种子发芽的处理方法[J]. 种子科技, 2006(2):48-49.
[17]	李季平, 古红梅, 吴诗光, 等. 聚乙二醇(PEG)处理对小麦萌发种子生理生化特性的影响[J]. 河南农业科学, 2002(6):4-6.
[18]	杨永青, 汪晓峰. 种子活力与生物膜的研究现状[J]. 植物学通报, 2004(6):641-648.
[19]	HEYDECKER W, HIGGINS J, GULLIVER R L. Accelerated germination by osmotic seed treatment[J]. Nature, 1973, 246:42-44. doi: 10.1038/246042a0 URL
[20]	汪少敏, 周红海, 胡铁军. 碘元素对提高水稻种子活性及发芽率的影响[J]. 中国稻米, 2019, 25(4):41-43.
[21]	王铁兵, 王鹏, 蒋建军, 等. 不同药剂引发处理对老化玉米种子萌发及幼苗生长的影响[J]. 中国草地学报, 2020, 42(5):31-39.

品种	指标	发芽势	发芽率	发芽指数	苗长/cm	活力指数	简易活力指数	根长/cm	苗重/g	根重/g
长7170	最小值	0.71	0.90	47.74	7.00	334.3	6.54	6.21	0.0497	0.0362
	最大值	0.88	0.95	68.45	10.02	647.55	9.23	8.18	0.0695	0.0561
	极差	0.17	0.05	20.71	3.02	313.25	2.69	1.97	0.0198	0.0199
	平均值	0.82	0.92	60.19	9.35	564.25	8.57	7.48	0.0644	0.0466
	标准差	0.05	0.02	5.73	0.92	82.37	0.81	0.6	0.0058	0.0063
	变异系数/%	5.75	1.74	9.52	9.82	14.60	9.39	8.09	8.96	13.43
长6388	最小值	0.71	0.89	46.9	7.36	343.38	6.55	5.03	0.0464	0.0295
	最大值	0.85	0.96	66.66	9.32	598.56	8.59	6.62	0.0573	0.0487
	极差	0.14	0.07	19.76	1.96	255.18	2.04	1.59	0.0109	0.0192
	平均值	0.79	0.92	59.79	8.08	485.21	7.42	5.73	0.0526	0.0361
	标准差	0.04	0.02	5.11	0.68	73.82	0.69	0.51	0.0038	0.0059
	变异系数/%	5.60	2.39	8.55	8.37	15.21	9.27	8.82	7.15	16.23

品种	指标	发芽势	发芽率	发芽指数	苗长/cm	活力指数	简易活力指数	根长/cm	苗重/g	根重/g
长7170	最小值	0.71	0.90	47.74	7.00	334.3	6.54	6.21	0.0497	0.0362
	最大值	0.88	0.95	68.45	10.02	647.55	9.23	8.18	0.0695	0.0561
	极差	0.17	0.05	20.71	3.02	313.25	2.69	1.97	0.0198	0.0199
	平均值	0.82	0.92	60.19	9.35	564.25	8.57	7.48	0.0644	0.0466
	标准差	0.05	0.02	5.73	0.92	82.37	0.81	0.6	0.0058	0.0063
	变异系数/%	5.75	1.74	9.52	9.82	14.60	9.39	8.09	8.96	13.43
长6388	最小值	0.71	0.89	46.9	7.36	343.38	6.55	5.03	0.0464	0.0295
	最大值	0.85	0.96	66.66	9.32	598.56	8.59	6.62	0.0573	0.0487
	极差	0.14	0.07	19.76	1.96	255.18	2.04	1.59	0.0109	0.0192
	平均值	0.79	0.92	59.79	8.08	485.21	7.42	5.73	0.0526	0.0361
	标准差	0.04	0.02	5.11	0.68	73.82	0.69	0.51	0.0038	0.0059
	变异系数/%	5.60	2.39	8.55	8.37	15.21	9.27	8.82	7.15	16.23

处理	发芽势	发芽率	发芽指数	苗长/cm	活力指数	简易活力指数	根长/cm	苗重/g	根重/g
1	0.71b	0.90a	47.74e	7.00c	334.30c	6.54c	6.20d	0.0497c	0.0431bcd
2	0.79ab	0.93a	54.27de	10.02a	542.70b	9.01a	6.78cd	0.0666a	0.0433bcd
3	0.87a	0.94a	68.44a	9.47a	647.55a	8.97a	7.85abc	0.0672a	0.0561a
4	0.83ab	0.92a	59.68bcd	9.67a	577.51ab	8.90a	8.18a	0.0695a	0.0540ab
5	0.81ab	0.90a	62.70abc	9.72a	609.78ab	8.82a	8.02a	0.0645ab	0.0522abc
6	0.83ab	0.90a	60.45abcd	10.02a	605.66ab	9.02a	7.72abc	0.0681a	0.0505abc
7	0.81ab	0.90a	58.05cd	9.75a	566.53ab	8.84a	7.52abc	0.0671a	0.0415cd
8	0.81ab	0.92a	59.67bcd	9.96a	594.45ab	9.23a	7.88ab	0.0679a	0.0438bcd
9	0.88a	0.93a	64.16abc	9.57a	614.22ab	8.93a	7.58abc	0.0669a	0.0419cd
10	0.81ab	0.90a	60.14abcd	9.37a	563.77ab	8.44ab	7.66abc	0.0617ab	0.0362d
11	0.87a	0.92a	66.74ab	8.27b	550.31b	7.58b	6.86bcd	0.0588b	0.0500abc

处理	发芽势	发芽率	发芽指数	苗长/cm	活力指数	简易活力指数	根长/cm	苗重/g	根重/g
1	0.71b	0.90a	47.74e	7.00c	334.30c	6.54c	6.20d	0.0497c	0.0431bcd
2	0.79ab	0.93a	54.27de	10.02a	542.70b	9.01a	6.78cd	0.0666a	0.0433bcd
3	0.87a	0.94a	68.44a	9.47a	647.55a	8.97a	7.85abc	0.0672a	0.0561a
4	0.83ab	0.92a	59.68bcd	9.67a	577.51ab	8.90a	8.18a	0.0695a	0.0540ab
5	0.81ab	0.90a	62.70abc	9.72a	609.78ab	8.82a	8.02a	0.0645ab	0.0522abc
6	0.83ab	0.90a	60.45abcd	10.02a	605.66ab	9.02a	7.72abc	0.0681a	0.0505abc
7	0.81ab	0.90a	58.05cd	9.75a	566.53ab	8.84a	7.52abc	0.0671a	0.0415cd
8	0.81ab	0.92a	59.67bcd	9.96a	594.45ab	9.23a	7.88ab	0.0679a	0.0438bcd
9	0.88a	0.93a	64.16abc	9.57a	614.22ab	8.93a	7.58abc	0.0669a	0.0419cd
10	0.81ab	0.90a	60.14abcd	9.37a	563.77ab	8.44ab	7.66abc	0.0617ab	0.0362d
11	0.87a	0.92a	66.74ab	8.27b	550.31b	7.58b	6.86bcd	0.0588b	0.0500abc

处理	发芽势	发芽率	发芽指数	苗长/cm	活力指数	简易活力指数	根长/cm	苗重/g	根重/g
1	0.71b	0.89b	46.90d	7.36d	343.38d	6.55b	5.03b	0.0464c	0.0295c
2	0.75ab	0.90ab	56.93c	7.51cd	479.35bc	6.75b	5.03b	0.0480c	0.0299c
3	0.85a	0.96a	66.66a	8.95ab	596.31a	8.59a	6.62a	0.0566ab	0.0321bc
4	0.84a	0.91ab	64.11ab	9.32a	598.56a	8.48a	5.75ab	0.0567b	0.0377bc
5	0.80ab	0.93ab	62.24abc	8.85ab	550.96ab	8.23a	5.57ab	0.0573a	0.0365bc
6	0.77ab	0.91ab	58.37bc	8.42b	438.47c	7.66b	6.09ab	0.0530abc	0.0300c
7	0.77ab	0.92ab	59.67bc	7.62cd	455.22c	7.01b	5.81ab	0.0561ab	0.0487a
8	0.80ab	0.91ab	58.72bc	7.73cd	454.07c	7.03b	5.60ab	0.0524abc	0.0414ab
9	0.76ab	0.95b	59.73bc	7.70cd	460.40c	7.31b	5.61ab	0.0503abc	0.0338bc
10	0.79ab	0.91ab	61.84abc	7.65cd	472.89c	6.96b	5.49ab	0.0523abc	0.0391bc
11	0.85a	0.91ab	62.57abc	7.80cd	487.66bc	7.10b	6.46a	0.0496bc	0.0383bc