Abstract: To reasonably analyze the local climate resources and their spatial distribution and serve Yuanjiang pitaya industry, based on the meteorological data and geographical information data (longitude and altitude, slope direction, gradient, etc.) from 1986 to 2015 in Yunnan, and through the methods of previous zoning experience, expert consultation, literature review, field research and discussion, we qualitatively judged the importance of meteorological factors to climate suitability or disaster risks, determined the climate suitability index and low-temperature damage index of pitaya; then, according to the zoning index elements, thin plate smoothing spline function’s statistical model was applied to insert the value for climatic element space; considering relevant geographical information data, we constructed the spatial raster data set, calculated and speculated the plantation suitability index and disaster risk index to classify the zoning results; and completed the refined agricultural climate zoning and low-temperature damage zoning of pitaya in Yuanjiang. The results indicated that: the plantation climate suitability area of pitaya was highly coincided with the low-temperature damage risk area; the zoning at both banks of the Yuanjiang River Valley included the eastern Lijiang Street, the middle Honghe Street, the southern Ganzhuang Street, and the eastern Manlai Street; the decision-making accuracy of developing pitaya industry was proved from the aspect of meteorological condition. This study provides a basis for planation development, industrial layout, prevention and mitigation of natural disasters for pitaya industry in Yuanjiang.