To select the suitable antitranspirant for facility cucumber, ‘Beinongjiaxiu’ and ‘Jinyou 35’ were used as materials, and a field experiment was conducted. Humic acid type Guoguang antitranspirant and film-forming Shenrun antitranspirant were selected, four treatments were set as Guoguang antitranspirant diluted 1000 times (T1), Guoguang antitranspirant diluted 500 times (T2), Shenrun antitranspirant diluted 20 times (T3), Shenrun antitranspirant diluted 40 times (T4), and water spray was taken as CK, to study the influence of different antitranspirants on the growth, biomass, yield and quality of cucumber. The results showed that compared with CK, for ‘Jinyou 35’, the SPAD value of leaves under T1, T2 and T3 increased by 4.8%, 7.4% and 9.4%, respectively, and the water content of underground part under T1, T2, T3 and T4 increased by 6.4, 17.2, 7.1 and 3.7 percentage points, respectively; the Vc content under T2 increased by 25.76% and the soluble protein increased by 11.54%. For ‘Beinongjiaxiu’, the SPAD value of leaves under T4 increased by 1.1% on average, the fresh weight of the aboveground part increased by 84.6%, the yield per plant increased by 27.2%, and the content of soluble protein increased by 50%. In general, 500 times dilution of Guoguang antitranspirant could be selected for ‘Jinyou 35’ cucumber, 40 times dilution of Shenrun antitranspirant could be selected for ‘Beinongjiaxiu’ cucumber.